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only those wells that contain Human PIINP
The concentration of Rabbit BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated RTN4 antibody
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present
Mouse ROBO4 (Roundabout Homolog 4) ELISA Kit TNFSF5 only those wells that containThe Mouse (ROBO4) Roundabout Homolog 4 ELISA Kit measures Roundabout Homolog 4 in Mouse samples. The plate has been pre coated with Mouse ROBO4 antibody. ROBO4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ROBO4 Antibody is added and binds to ROBO4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ROBO4 antibody. After incubation unbound Streptavidin HRP is washed away
